LOCUS       40924_23634       11591 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB671 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Momtaz S, Dutta A, Tanaka Y, Aboulela M, Nishimura K, Sugiura S, 
            Niwa T, Maeo K, Goto-Yamada S, Kimura T, Ishiguro S, Mano S, 
            Nakagawa T.
  TITLE     Gateway Binary Vectors with Organelle-Targeted Fluorescent Proteins 
            for Highly Sensitive Reporter Assay in Gene Expression Analysis of 
            Plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11591)
  AUTHORS   Momtaz S, Dutta A, Tanaka Y, Aboulela M, Nishimura K, Sugiura S, 
            Niwa T, Maeo K, Goto-Yamada S, Kimura T, Ishiguro S, Mano S, 
            Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2018) Contact:Tsuyoshi Nakagawa Shimane 
            University, Interdisciplinary Center for Science Research; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 11591)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11591)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2018) Contact:Tsuyoshi Nakagawa Shimane University, 
            Interdisciplinary Center for Science Research; 1060 Nishikawatsu, 
            Matsue, Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1930..2054)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2103..2114)
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     CDS             2236..2952
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     terminator      2977..3229
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3263..3279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3287..3303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3311..3341)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3356..3377)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3413..3665)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3828..4376)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4428..4607)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5113..5137)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5658..6446
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6695..7283
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7469..7609)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7953..8147)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8216..9286)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9718..10344)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcatggca atggctgttt tccgtcgcga agggaggcgt
     2101 ctcctccctt caatcgccgc tcgcccaatc gctgctatcc gatctcctct ctcttctgac
     2161 caggaggaag gacttcttgg agttcgatct atctcaactc aagtggtgcg taaccgcatg
     2221 aagagtgtta agaacatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     2281 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     2341 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     2401 gtgccctggc ccaccctcgt gaccaccttc acctacggcg tgcagtgctt cagccgctac
     2461 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     2521 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     2581 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2641 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     2701 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     2761 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     2821 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     2881 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactca cggcatggac
     2941 gagctgtaca agtaagctta gagctcgaat ttccccgatc gttcaaacat ttggcaataa
     3001 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     3061 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     3121 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     3181 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattggtt
     3241 ccggaaccaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     3301 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     3361 tgagctaact cacattaggc tgaattaggc gcgcctattt ctattaattc ccgatctagt
     3421 aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct
     3481 atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac
     3541 gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata
     3601 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
     3661 cgatcgggga aattcgagct ggggacgtcc ccagctcggt accccctcct gggcacggtc
     3721 gtacgcgatc cgccgcggtt ccacctcacc attgcgggta tgccagttga gttcgatgag
     3781 ttcccgtacc agctccaact cgggcgatgc cacgcgcact ccgttcagat ctcggtgacg
     3841 ggcaggaccg gacggggcgg taccggcagg ctgaagtcca gctgccagaa acccacgtca
     3901 tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc cgcggggggc atatccgagc
     3961 gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga tgacagcgac cacgctcttg
     4021 aagccctgtg cctccaggga cttcagcagg tgggtgtaga gcgtggagcc cagtcccgtc
     4081 cgctggtggc ggggggagac gtacacggtc gactcggccg tccagtcgta ggcgttgcgt
     4141 gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc cgtccacctc ggcgacgagc
     4201 cagggatagc gctcccgcag acggacgagg tcgtccgtcc actcctgcgg ttcctgcggc
     4261 tcggtacgga agttgaccgt gcttgtctcg atgtagtggt tgacgatggt gcagaccgcc
     4321 ggcatgtccg cctcggtggc acggcggatg tcggccgggc gtcgttctgg gctcatatct
     4381 tattgccccc cgggatcaga tctggattga gagtgaatat gagactctaa ttggataccg
     4441 aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc tagctgatag
     4501 tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc ttagctcatt
     4561 aaactccaga aacccgcggc tgagtggctc cttcaacgtt gcggttctgt cagttccaaa
     4621 cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc ttaattctcc
     4681 gctcatgatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt
     4741 actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt
     4801 gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc
     4861 tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg
     4921 ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg
     4981 cgccctgagt gcttgcggca gcgtgaagct gatccgtcga gattaataga aataaattca
     5041 gcctaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct
     5101 aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga
     5161 ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt
     5221 cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag
     5281 aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga
     5341 ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat
     5401 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     5461 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     5521 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     5581 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     5641 aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag
     5701 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     5761 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     5821 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     5881 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     5941 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     6001 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     6061 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     6121 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     6181 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     6241 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     6301 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     6361 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     6421 gcgagatcac caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc
     6481 cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc
     6541 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca
     6601 aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6661 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6721 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6781 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6841 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6901 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6961 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7021 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7081 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7141 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7201 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7261 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7321 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7381 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7441 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     7501 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     7561 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     7621 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     7681 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     7741 cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc
     7801 cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga
     7861 taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt
     7921 gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag
     7981 ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag
     8041 tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc
     8101 ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt
     8161 ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac
     8221 cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct
     8281 gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca
     8341 cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga
     8401 acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc
     8461 cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc
     8521 cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc
     8581 cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca
     8641 ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc
     8701 gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact
     8761 tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc
     8821 ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc
     8881 acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt
     8941 agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca
     9001 tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt
     9061 gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg
     9121 attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct
     9181 cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca
     9241 gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg
     9301 cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca
     9361 accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt
     9421 ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt
     9481 actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta
     9541 ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca
     9601 tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac
     9661 ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat
     9721 gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg
     9781 ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt
     9841 gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc
     9901 gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt
     9961 gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg
    10021 gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc
    10081 cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc
    10141 gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc
    10201 cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc
    10261 gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg
    10321 gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc
    10381 atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt
    10441 agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca
    10501 tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg
    10561 aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg
    10621 tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg
    10681 ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg
    10741 tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa
    10801 gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg
    10861 cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg
    10921 cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg
    10981 cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca
    11041 ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg
    11101 tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt
    11161 caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca
    11221 gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg
    11281 tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc
    11341 tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc
    11401 aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt
    11461 cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga
    11521 tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa
    11581 cggataaacc t
//
